Understanding and addressing concerns from Japanese customers about changes in the gluten properties of CWRS wheat exposed to heat stress
Bread Wheat and Durum Research Program
Dr. Bin Xiao Fu
Research scientist/program manager
Bread Wheat and Durum Research
binxiao.fu@grainscanada.gc.ca
Team members
Research scientist/program manager
- Dr. Bin Xiao Fu
Chemists
- Carly Isaak
- Dr. Kun Wang
- Dr. Ray Bacala
Technicians
- Altash Yirdaw
- Angelique Parajas
- Andrea Iverson
- Dale Taylor
- Dylan Lloyd (term)
- Jennifer Nguyen (term)
- Joseffus Santos (on assignment)
- Katherine Cordova (on assignment)
- Shermy Jayasekara
- Yuming Chen
The Bread Wheat and Durum Research Program supports the quality assurance system for Canadian wheat. We investigate the impact of various grading factors on functionality to provide the scientific basis for their tolerances in wheat classes and grades. We also analyze the quality of new crops and monitor wheat cargos and evaluate new wheat varieties recommended for registration for designation into Canadian wheat classes based on their merit. Our research focuses on understanding how the physicochemical and biochemical properties of wheat influence its quality and developing new techniques for evaluating wheat quality.
Dough qualities of CWRS wheat exposed to heat stress
We recently started a collaboration with the milling and baking industry in Japan, which is the largest customer of No. 1 Canada Western Red Spring (CWRS) wheat. This was done to address concerns that Japanese bakers had about the long mixing requirements and low extensibility of dough made from CWRS wheat grown in the hot and dry years of 2021 and 2023. They described the dough as “strong but brittle and inextensible” and reported that their processes had to be adjusted. This resulted in increased production costs and an undesirable variation in end product quality.
We compared the results of our Extensograph and Farinograph tests from 2020 to 2023 and confirmed that doughs made from CWRS wheat exposed to heat stress were significantly stronger, as indicated by higher maximum resistance (Rmax) values, and longer development and stability times (Table 1). The extensibility of dough was similar, however, for all four years. Our standard test methods, widely used internationally, did not capture the brittle and inextensible dough properties described by Japanese customers.
Does gluten protein composition change under heat stress?
To better understand the effect of heat stress on dough, we fractionated the gluten proteins in flour samples of CWRS wheat from 2020 to 2023 into insoluble glutenin, soluble glutenin and monomeric proteins. Insoluble glutenin contributes to dough strength and the latter two fractions contribute to the extensibility of dough. The results show a higher ratio of insoluble glutenin to soluble glutenin in wheat exposed to heat stress (Figure 1), which is consistent with a stronger dough and longer mixing time. However, the brittle and inextensible dough properties are not well explained by the distribution of gluten protein fractions.
Assessment of commercial flour samples from Japan
We then assessed six commercial flour samples, milled mostly from CWRS wheat, that were provided by a major wheat milling company in Japan. Our collaborator reported that the dough properties of the samples ranged from well balanced to brittle and inextensible. We found no significant differences in extensibility between the six samples, however, using our standard test methods (Table 2).
Technical exchange with Japan
An in-person technical exchange was arranged with Japanese experts who have extensive experience in assessing dough handling properties. Our Sponge and Dough bake method was compared with the Shokupan bake method used in Japan and some notable differences in formulation and methodology were observed. With input from the Japanese experts, the Shokupan bake method was adapted for use with our equipment and our staff were trained in the subjective scoring system used in Japan to assess dough qualities. For example, extensibility is evaluated by how far the dough stretches before tearing and thinly stretched dough is considered brittle if it breaks easily when lightly touched. In a blind re-test of the Japanese commercial flour samples using the Shokupan bake method, our staff were able to differentiate the dough properties important to the Japanese baking industry (Figure 2).
We also evaluated the Shokupan dough properties of the 2023 CWRS wheat samples received by the Harvest Sample Program and the results were consistent with the description of dough made from 2023 wheat cargos received by Japan.
Next steps
With the cooperation of our collaborator, we will continue receiving samples of commercial flour from Japan with good, average, and poor dough properties to be used as references to continue the training of our staff and for the evaluation of cargo and new crop No. 1 CWRS wheat aggregates. We also plan to conduct research to understand the biochemical basis of the brittle and inextensible dough properties that result from heat stress. Gluten consists of many types of proteins and their size distribution, relative proportions, cross-linking capability, hydrophobicity, and various protein-protein interactions all contribute to gluten viscoelasticity. Although these properties are mostly controlled by genetics, they can also be significantly modified by heat stress. Knowledge at the molecular level is critical for the selection or development of wheat varieties with improved consistency in gluten functionality under heat or other environmental stresses.
Graph data
| Crop year | Ratio of IG/SG | Ratio of IG/(MP + SG) |
|---|---|---|
| 2020 | 0.697 | 0.220 |
| 2021 | 1.020 | 0.263 |
| 2022 | 0.830 | 0.254 |
| 2023 | 0.960 | 0.272 |
Table 1 Properties of dough made with CWRS wheat from 2020 to 2023Footnote 1
| Dough property | 2020 | 2021Footnote 2 | 2022 | 2023Footnote 2 |
|---|---|---|---|---|
| Rmax, BU | 552 | 712 | 631 | 796 |
| Extensibility, cm | 19.8 | 19.1 | 20.1 | 18.6 |
| Area, cm2 | 137 | 169 | 160 | 183 |
| Dough property | 2020 | 2021Footnote 2 | 2022 | 2023Footnote 2 |
|---|---|---|---|---|
| Absorption, % | 65 | 65 | 65 | 65 |
| Development time, min | 6.3 | 10.8 | 7.3 | 8.8 |
| Stability, min | 11.5 | 21.0 | 14.5 | 25.8 |
Table 2 Properties of dough made with commercial flour samples from Japan
| Sample 1 | Sample 2 | Sample 3 | Sample 4 | Sample 5 | Sample 6 | |
|---|---|---|---|---|---|---|
| Characteristics reported by Japanese collaborator | Well balanced | Balanced; slightly lower in extensibility | Strong but insufficient extensibility | Strong but brittle and inextensible | Fragile, low extensibility | Weak, brittle and inextensible |
| Dough properties | Sample 1 | Sample 2 | Sample 3 | Sample 4 | Sample 5 | Sample 6 |
|---|---|---|---|---|---|---|
| Rmax, BU | 906 | 907 | 964 | 892 | 950 | 803 |
| Extensibility, cm | 15.3 | 16.1 | 14.9 | 16.7 | 15.1 | 17.2 |
| Area, cm2 | 168 | 177 | 170 | 183 | 173 | 172 |
| Dough properties | Sample 1 | Sample 2 | Sample 3 | Sample 4 | Sample 5 | Sample 6 |
|---|---|---|---|---|---|---|
| Absorption, % | 64.0 | 64.6 | 64.1 | 64.0 | 63.5 | 63.6 |
| Development time, min | 17.0 | 17.3 | 18.0 | 14.3 | 17.5 | 13.3 |
| Stability, min | 20.0 | 22.0 | 25.0 | 23.0 | 23.0 | 21.0 |
Recent publications
- Bacala, R., Hatcher, D.W., Perreault, H., and B.X. Fu. 2023. Partial C-terminal truncation of Bx and Dy high molecular weight glutenin subunits after conserved aspartate. J. Cereal Sci. 114:103805. https://doi.org/10.1016/j.jcs.2023.103805
- Wang, K., Taylor, D., Ruan Y., Pozniak, C.J., Izydorczyk, M. and B.X. Fu. 2023. Unveiling the factors affecting milling quality of durum wheat: influence of kernel physical properties, grain morphology and intrinsic milling behaviours. J. Cereal Sci. 113: 103755. https://doi.org/10.1016/j.jcs.2023.103755
- Iwaki, S, Fu, B.X., and K. Hayakawa. 2023. Behavior of protein aggregates via electrostatic interactions or hydrogen bonds during dough formation. J. Cereal Sci. 111: 103683. https://doi.org/10.1016/j.jcs.2023.103683
- Tittlemier, S.A., Bestvater, L., Chan, J., Timofeiev, V., Richter, A., Wang, K., Ruan, Y., Izydorczyk, M., and B.X. Fu. 2023. Diverging fates of cadmium and glyphosate during pasta cooking. Food Addit. Contam. Part A 40(11) 1459-1469. https://doi.org/10.1080/19440049.2023.2264976


